Abstract
Piwi-interacting RNAs (piRNA) are fully modified by 2′-O-methylation at their 3′-termini. This terminal methylation is required to prevent 3′-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2′-O-methylation at 3′-termini of piRNAs.
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Acknowledgments
We sincerely appreciate the Suzuki laboratory members for fruitful discussion on this project. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan (to Takeo S. and Tsutomu S.) and by a grant from the New Energy and Industrial Technology Development Organization (NEDO) (to Tsutomu S.).
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Suzuki, T., Miyauchi, K., Sakaguchi, Y., Suzuki, T. (2014). Biochemical and Mass Spectrometric Analysis of 3’-End Methylation of piRNAs. In: Siomi, M. (eds) PIWI-Interacting RNAs. Methods in Molecular Biology, vol 1093. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-694-8_6
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DOI: https://doi.org/10.1007/978-1-62703-694-8_6
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