Abstract
Current baculovirus expression vector systems (BEVS) rely on either using homologous recombination or site specific transposition (Tn7 transposition) to obtain recombinant baculovirus. Each approach has its own merits. To date, the choice of transfer plasmids limited expression of target proteins to only one of the two types of BEVS. Here we describe OmniBac, comprising novel universal multigene transfer plasmids that can access all BEVS currently in use for protein production in the community. Detailed protocols are presented for integrating OmniBac plasmids into baculoviral genomes used for heterologous protein production in insect cells.
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Acknowledgements
We thank all members of the Berger laboratory for helpful discussions. This work was supported by the Centre National de Recherche Scientifique (CNRS) through a PEPS discovery grant (to IB), and by the European Commission (EC) Framework Program 7 (PCUBE, BioSTRUCT-X and ComplexINC, to IB). DBTGR is recipient of an EC/EMBL CoFund EIPOD fellowship.
Competing Financial Interest Statement
The authors declare competing financial interests. IB is the author on patents or patent applications detailing reagents and parts of the methods here described.
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Thimiri Govinda Raj, D.B., Vijayachandran, L.S., Berger, I. (2014). OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems. In: Chen, Y. (eds) Structural Genomics. Methods in Molecular Biology, vol 1091. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-691-7_7
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DOI: https://doi.org/10.1007/978-1-62703-691-7_7
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