Abstract
While steady-state 13C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state 13C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.
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References
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Acknowledgments
S.K.M. acknowledges financial support from the University of Oxford (Clarendon Scholarship), Exeter College (Mr Krishna Pathak Scholarship) and a UK Overseas Research Student award. We thank W. Wiechert (Forschungszüentrum Jülich GmbH) for permission to use 13C-FLUX and P. Spelluci (Fachbereich Mathematik, Technische Universität Darmstadt, Germany) for developing the Donlp2 algorithm.
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Kruger, N.J., Masakapalli, S.K., Ratcliffe, R.G. (2014). Optimization of Steady-State 13C-Labeling Experiments for Metabolic Flux Analysis. In: Dieuaide-Noubhani, M., Alonso, A. (eds) Plant Metabolic Flux Analysis. Methods in Molecular Biology, vol 1090. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-688-7_4
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DOI: https://doi.org/10.1007/978-1-62703-688-7_4
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