Abstract
The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ratcliffe RG, Shachar-Hill Y (2001) Probing plant metabolism with NMR. Annu Rev Plant Physiol Plant Mol Biol 52:499–526
Troufflard S, Roscher A, Thomasset B et al (2007) In vivo 13C-NMR determines metabolic fluxes and steady state in linseed embryos. Phytochem 68:2341–2350
Noack S, Nöh K, Moch M et al (2011) Stationary versus non-stationary 13C-MFA: a comparison using a consistent dataset. J Biotechnol 154:179–190
Fan TW-M, Higashi RM, Lane AN (1986) Monitoring of hypoxic metabolism in superfused plant tissues by in vivo 1H NMR. Arch Biochem Biophys 251:674–687
Ratcliffe RG, Roscher A, Shachar-Hill Y (2001) Plant NMR spectroscopy. Prog Nucl Magn Reson Spectrosc 39:267–300
Massou S, Nicolas C, Letisse F et al (2007) Application of 2D-TOCSY NMR to the measurement of specific 13C-enrichments in complex mixtures of 13C-labeled metabolites. Metab Eng 9:252–257
Shachar-Hill Y, Pfeffer PE, Germann MW (1996) Following plant metabolism in vivo and in extracts with heteronuclear two-dimensional nuclear magnetic resonance spectroscopy. Anal Biochem 243:110–118
Fan TW-M (1996) Recent advances in profiling plant metabolites by multi-nuclear and multi-dimensional NMR. In: Shachar-Hill Y, Pfeffer PE (eds) Nuclear magnetic resonance in plant biology. American Society of Plant Physiologists, Rockville, MD, USA, pp 181–254
Roscher A, Emsley L, Raymond P et al (1998) Unidirectional steady-state rates of central metabolism enzymes measured simultaneously in a living tissue. J Biol Chem 273:25053–25061
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Roscher, A., Troufflard, S., Taghki, A.I. (2014). In Vivo NMR for 13C metabolic Flux Analysis. In: Dieuaide-Noubhani, M., Alonso, A. (eds) Plant Metabolic Flux Analysis. Methods in Molecular Biology, vol 1090. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-688-7_10
Download citation
DOI: https://doi.org/10.1007/978-1-62703-688-7_10
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-687-0
Online ISBN: 978-1-62703-688-7
eBook Packages: Springer Protocols