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Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents

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Exocytosis Methods

Part of the book series: Neuromethods ((NM,volume 83))

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Abstract

Regulated exocytosis is a fundamental event in specialized secretory organs that has been primarily studied in in vitro and ex vivo model systems. The recent application of intravital microscopy to image subcellular structures in vivo has enabled researchers to investigate the machinery controlling regulated exocytosis in live rodents. Here, we describe selected experimental models that have been used to investigate the dynamics of the secretory granules after their initial fusion their with the plasma membrane. Specifically, we used rodent salivary glands, an established model for exocrine secretion. Our goal is to provide the reader with guidelines on how to apply both qualitative and quantitative intravital microscopy to study regulated exocytosis and to highlight advantages and limitations of this approach.

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Acknowledgments

This research was supported by the Intramural Research Program of the NIH, National Institute of Dental, and Craniofacial Research.

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Authors and Affiliations

  1. Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA

    Oleg Milberg, Natalie Porat-Shliom, Muhibullah Tora, Laura Parente, Andrius Masedunskas & Roberto Weigert

  2. Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ, USA

    Oleg Milberg

  3. Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA

    Oleg Milberg

Authors
  1. Oleg Milberg
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  2. Natalie Porat-Shliom
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  3. Muhibullah Tora
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  4. Laura Parente
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  5. Andrius Masedunskas
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  6. Roberto Weigert
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Editors and Affiliations

  1. School of Biomedical Sciences, University of Queensland, St. Lucia, Queensland, Australia

    Peter Thorn

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Milberg, O., Porat-Shliom, N., Tora, M., Parente, L., Masedunskas, A., Weigert, R. (2014). Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents. In: Thorn, P. (eds) Exocytosis Methods. Neuromethods, vol 83. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-676-4_8

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  • DOI: https://doi.org/10.1007/978-1-62703-676-4_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-675-7

  • Online ISBN: 978-1-62703-676-4

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