Abstract
UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high-resolution second derivative absorption methods can also provide information concerning peptide dynamics. Data from several low-resolution spectroscopic techniques, including UV absorption, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
Mangala R. Liyanage and Kunal Bakshi have contributed equally to this manuscript.
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Liyanage, M.R., Bakshi, K., Volkin, D.B., Middaugh, C.R. (2014). Ultraviolet Absorption Spectroscopy of Peptides. In: Nixon, A. (eds) Therapeutic Peptides. Methods in Molecular Biology, vol 1088. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-673-3_15
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DOI: https://doi.org/10.1007/978-1-62703-673-3_15
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