Abstract
Vpx is coded almost exclusively by members of the SIVSM/HIV-2 lineage of primate lentiviruses, it is incorporated into virion particles and is thus present during the early phases of infection of target cells. While Vpx exerts no detectable function during the infection of most cell types, it potently counteracts a cellular restriction that targets incoming lentiviruses specifically in myeloid cells. As a consequence of this function, Vpx improves the efficiency of lentiviral infection of dendritic cells (DCs), macrophages, and monocytes. Here, we describe how the positive function exerted by Vpx during the early phases of infection of myeloid cells can be used to augment the efficiency of lentiviral vector-mediated gene transfer in these cells.
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Acknowledgments
Work in our laboratory is supported by grants from the Agence Nationale de Recherche sur le SIDA (ANRS), SIDACTION, Fondation pour la Recherche Médicale (FRM), INSERM, and Ecole Normale Supérieure de Lyon (ENS-Lyon). A.C. is supported by the Centre National de la Recherche Sciéntifique (CNRS).
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Berger, G., Cimarelli, A. (2014). SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells. In: Vicenzi, E., Poli, G. (eds) Human Retroviruses. Methods in Molecular Biology, vol 1087. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-670-2_13
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DOI: https://doi.org/10.1007/978-1-62703-670-2_13
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-669-6
Online ISBN: 978-1-62703-670-2
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