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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols pp 103–111Cite as

Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1085))

Abstract

Pulsed-field gel electrophoresis (PFGE) is the most applied and effective genetic typing method for epidemiological studies and investigation of foodborne outbreaks caused by different pathogens, including Staphylococcus aureus. The technique relies on analysis of large DNA fragments generated by the cleavage of intact bacterial chromosomes with a rare cutting restriction enzyme, subsequently resolved by pulsed-field electrophoresis with periodic changes of the orientation of the electrical field across the gel. The high discriminatory power, improved reproducibility due to standardization of experimental protocols and data interpretation guidelines, and establishment of a national PFGE database of S. aureus profiles have made it a valuable means for global tracking of S. aureus infection sources and determination of genetic relatedness of outbreak isolates.

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Author information

Authors and Affiliations

  1. Molecular Characterization of Foodborne Pathogens Research Unit, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA, USA

    Yiping He

  2. Molecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, Wyndmoor, PA, USA

    Yanping Xie & Sue Reed

Authors
  1. Yiping He
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  2. Yanping Xie
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  3. Sue Reed
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Editor information

Editors and Affiliations

  1. Dept. Veterinary & Biomedical Sciences, University of Minnesota College of Veterinary Medicine, St. Paul, Minnesota, USA

    Yinduo Ji

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© 2014 Springer Science+Business Media, LLC

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He, Y., Xie, Y., Reed, S. (2014). Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 1085. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-664-1_6

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  • DOI: https://doi.org/10.1007/978-1-62703-664-1_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-663-4

  • Online ISBN: 978-1-62703-664-1

  • eBook Packages: Springer Protocols

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