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The Cre/Lox System to Assess the Development of the Mouse Brain

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Brain Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1082))

Abstract

Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circumvented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.

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Acknowledgements

Work in F.M.R. laboratory in Friedrich Miescher Institute (Basel, Switzerland) is supported by the Swiss National Science Foundation (Sinergia CRSI33_127440), ARSEP, and the Novartis Research Foundation.

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Authors and Affiliations

  1. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

    Claudius F. Kratochwil & Filippo M. Rijli

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  1. Claudius F. Kratochwil
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  2. Filippo M. Rijli
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Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G Sprecher

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Kratochwil, C.F., Rijli, F.M. (2014). The Cre/Lox System to Assess the Development of the Mouse Brain. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 1082. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-655-9_20

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  • DOI: https://doi.org/10.1007/978-1-62703-655-9_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-654-2

  • Online ISBN: 978-1-62703-655-9

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