Abstract
Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. “Dynamic neuroanatomy” defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy.
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Acknowledgements
We thank the generosity of C.B. Chien for the Tol2kit and advice and dedicate this article to his memory. We also thank T. Zimmermann and J. Swoger for advice on, respectively, confocal and SPIM microscopy. The original research that encouraged the development of this methodology was supported by a grant from the European Research Council (ERC-2007-StG SENSORINEURAL) and by the Ministerio de Ciencia e Innovación of Spain to H.L. S.A.F. was supported by an Intra-European Marie Curie Fellowship from the European Union.
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Faucherre, A., López-Schier, H. (2014). Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 1082. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-655-9_13
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DOI: https://doi.org/10.1007/978-1-62703-655-9_13
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