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Brain Development pp 143–154Cite as

Microinjection Manipulations in the Elucidation of Xenopus Brain Development

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1082))

Abstract

Microinjection has a long and distinguished history in Xenopus and has been used to introduce a surprisingly diverse array of agents into embryos by both intra- and intercellular means. In addition to nuclei, investigators have variously injected peptides, antibodies, biologically active chemicals, lineage markers, mRNA, DNA, morpholinos, and enzymes. While enumerating many of the different microinjection approaches that can be taken, we will focus upon the mechanical operations and options available to introduce mRNA, DNA, and morpholinos intracellularly into early stage embryos for the study of neurogenesis.

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Acknowledgment

M.J.C. is supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada Grant #203549.

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Authors and Affiliations

  1. Biological Sciences, University of Windsor, Windsor, ON, Canada

    Cristine Smoczer, Lara Hooker, Saqib S. Sachani & Michael J. Crawford

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  1. Cristine Smoczer
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  2. Lara Hooker
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  3. Saqib S. Sachani
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  4. Michael J. Crawford
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Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G Sprecher

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Smoczer, C., Hooker, L., Sachani, S.S., Crawford, M.J. (2014). Microinjection Manipulations in the Elucidation of Xenopus Brain Development. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 1082. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-655-9_10

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  • DOI: https://doi.org/10.1007/978-1-62703-655-9_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-654-2

  • Online ISBN: 978-1-62703-655-9

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