Abstract
This chapter describes an experimental layout for time and spectrally resolved fluorescence measurements with femtosecond time resolution based on Kerr gating. The combination of data recorded using different Kerr media allows a temporal dynamic range from ~100 fs to several nanoseconds. Simultaneous analysis of multiple datasets is described.
Key words
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Valeur B (2002) Molecular fluorescence: principles and applications. Wiley-VCH, Weinheim
Laptenok SP, van Stokkum IHM, Borst JW et al (2012) Disentangling picosecond events that complicate the quantitative use of the calcium sensor YC3.60. J Phys Chem B 116:3013–3020
van Oort B, Eremeeva EV, Koehorst RBM et al (2009) Picosecond fluorescence relaxation spectroscopy of the calcium-discharged photoproteins aequorin and obelin. Biochemistry 48:10486–10491
van Stokkum IHM, van Oort B, van Mourik F et al (2008) (Sub)-picosecond spectral evolution of fluorescence studied with a synchroscan streak-camera system and target analysis. In: Aartsma TJ, Matysik J (eds) Biophysical techniques in photosynthesis, vol II. Springer, Dordrecht, pp 223–240
Cannizzo A, Bräm O, Zgrablic G et al (2007) Femtosecond fluorescence upconversion setup with broadband detection in the ultraviolet. Opt Lett 32:3555–3557
Zhang X-X, Würth C, Zhao L et al (2011) Femtosecond broadband fluorescence upconversion spectroscopy: improved setup and photometric correction. Rev Sci Instrum 82:063108
Schanz R, Kovalenko SA, Kharlanov V et al (2001) Broad-band fluorescence upconversion for femtosecond spectroscopy. Appl Phys Lett 79:566–568
Nakamura R, Kanematsu Y (2004) Femtosecond spectral snapshots based on electronic optical Kerr effect. Rev Sci Instrum 75:636–645
Schmidt B, Laimgruber S, Zinth W et al (2003) A broadband Kerr shutter for femtosecond fluorescence spectroscopy. Appl Phys B 76:809–814
Arzhantsev S, Maroncelli M (2005) Design and characterization of a femtosecond fluorescence spectrometer based on optical Kerr gating. Appl Spectrosc 59:206–226
Duguay MA (1969) An ultrafast light gate. Appl Phys Lett 15:192–194
Duguay MA, Hansen JW (1969) Direct measurement of picosecond lifetimes. Opt Commun 1:254–256
Cerullo G, de Silvestri S (2003) Ultrafast optical parametric amplifiers. Rev Sci Instrum 74:1–18
Yan L, Si J, Yan Y et al (2011) Pump power dependence of femtosecond two-color optical Kerr shutter measurements. Opt Express 19:11196–11201
Weigel A, Pfaffe M, Sajadi M et al (2012) Barrierless photoisomerisation of the “simplest cyanine”: joining computational and femtosecond optical spectroscopies to trace the full reaction path. Phys Chem Chem Phys 14:13350–13364
van Stokkum IHM, Larsen DS, van Grondelle R (2004) Global and target analysis of time-resolved spectra. Biochim Biophys Acta 1657:82–104
van Wilderen LJGW, Lincoln CN, van Thor JJ (2011) Modelling multi-pulse population dynamics from ultrafast spectroscopy. PLoS One 6:e17373
Snellenburg JJ, Laptenok SP, Seger R et al (2012) Glotaran: a Java—based graphical user interface for the R-package TIMP. J Stat Soft 49:1–23
Roth NJL, Craig AC (1974) Predicted observable fluorescent lifetimes of several cyanines. J Phys Chem 78:1154–1155
Nuernberger P, Vogt G, Gerber G et al (2006) Femtosecond study on the isomerization dynamics of NK88. I. Ground-state dynamics after photoexcitation. J Chem Phys 125:44512
Vogt G, Nuernberger P, Gerber G et al (2006) Femtosecond study on the isomerization dynamics of NK88. II. Excited-state dynamics. J Chem Phys 125:44513
Pal MK, Ghosh JK (1994) Energy transfer and formation of exciplex between thiacyanine and acridine orange facilitated by anionic biopolymers and synthetic polymers. J Photochem Photobiol A 78:31–37
Thomas MS, Nuñez V, Upadhyayula S et al (2010) Kinetics of bacterial fluorescence staining with 3,3′-diethylthiacyanine. Langmuir 26:9756–9765
Mammone JF, Sharma SK, Nicol M (1980) Raman spectra of methanol and ethanol at pressures up to 100 kbar. J Phys Chem 84:3130–3134
Brückner C, Notni G, Tünnermann A (2010) Optimal arrangement of 90° off-axis parabolic mirrors in THz setups. Optik (Jena) 121:113–119
Schmidt A, Chiesa M, Chen X et al (2008) An optical pump-probe technique for measuring the thermal conductivity of liquids. Rev Sci Instrum 79:064902
Ganeev RA, Ryasnyanskiĭ AI, Kuroda H (2006) Nonlinear optical characteristics of carbon disulfide. Opt Spectrosc 100:108–118
Thomsen CL, Madsen D, Thøgersen J et al (1999) Femtosecond spectroscopy of the dissociation and geminate recombination of aqueous CS2. J Chem Phys 111:703
Press WH, Teukolsky SA, Vetterling WT et al (2007) Numerical recipes 3rd Edition: the art of scientific computing. Cambridge University Press, Cambridge
Golub GH, Van Loan CF (1996) Matrix computations, 3rd edn. The Johns Hopkins University Press, Baltimore
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Laptenok, S.P., Nuernberger, P., Lukacs, A., Vos, M.H. (2014). Subpicosecond Kerr-Gate Spectrofluorometry. In: Engelborghs, Y., Visser, A. (eds) Fluorescence Spectroscopy and Microscopy. Methods in Molecular Biology, vol 1076. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-649-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-62703-649-8_13
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-648-1
Online ISBN: 978-1-62703-649-8
eBook Packages: Springer Protocols