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Upconversion Spectrophotofluorometry

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Book cover Fluorescence Spectroscopy and Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1076))

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Abstract

As the other chapters attest, sensitivity of fluorescent molecules to their local environment has created powerful tools in the study of molecular biology, particularly in the study of protein, DNA, and lipid dynamics. Surprisingly, even events faster than the nanosecond lifetimes of fluorophores are important in protein function, and in particular, events lasting just a few ps reflect on water motion and the coupled dynamics of proteins. These ultrafast phenomena can best be studied by using the same laser that excites fluorescence to also “strobe” the emission, providing sub-picosecond time slices of the action. We explain the strobing “upconversion” technique and some limits on its execution.

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Author information

Authors and Affiliations

  1. NIDDK, National Institutes of Health, Bethesda, MD, USA

    Arianna Biesso

  2. NHLBI, National Institutes of Health, Bethesda, MD, USA

    Jianhua Xu & Jay R. Knutson

Authors
  1. Arianna Biesso
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  2. Jianhua Xu
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  3. Jay R. Knutson
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Editor information

Editors and Affiliations

  1. KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium

    Yves Engelborghs

  2. MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands

    Antonie J.W.G. Visser

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Biesso, A., Xu, J., Knutson, J.R. (2014). Upconversion Spectrophotofluorometry. In: Engelborghs, Y., Visser, A. (eds) Fluorescence Spectroscopy and Microscopy. Methods in Molecular Biology, vol 1076. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-649-8_12

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  • DOI: https://doi.org/10.1007/978-1-62703-649-8_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-648-1

  • Online ISBN: 978-1-62703-649-8

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