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Nanometrology

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Fluorescence Spectroscopy and Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1076))

Abstract

Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

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Acknowledgements

The authors wish to thank the EPSRC and SFC for research support and SUPA, NPL, and Horiba Jobin Yvon IBH Ltd for a studentship held by PY.

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Authors and Affiliations

  1. The Photophysics Research Group, Department of Physics, Centre for Molecular Nanometrology, SUPA, University of Strathclyde, Glasgow, Scotland, UK

    David J. S. Birch & Philip Yip

Authors
  1. David J. S. Birch
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  2. Philip Yip
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Editor information

Editors and Affiliations

  1. KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium

    Yves Engelborghs

  2. MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands

    Antonie J.W.G. Visser

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© 2014 Springer Science+Business Media, LLC

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Birch, D.J.S., Yip, P. (2014). Nanometrology. In: Engelborghs, Y., Visser, A. (eds) Fluorescence Spectroscopy and Microscopy. Methods in Molecular Biology, vol 1076. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-649-8_11

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  • DOI: https://doi.org/10.1007/978-1-62703-649-8_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-648-1

  • Online ISBN: 978-1-62703-649-8

  • eBook Packages: Springer Protocols

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