Abstract
Sequential replica method facilitates in vivo imaging of plant surface and provides data sufficient for detailed computation of geometry and growth. It enables obtaining a series of high-resolution images visualizing details of the examined surface. Series of molds, made in dental polymer, representing the examined surface are used to obtain casts in epoxy resin, which are in turn observed by scanning electron microscopy, while the structure itself remains intact. Images obtained from casts can be further used for data extraction, comprising 3D reconstruction and computation of local geometry and cell growth parameters. The sequential replica method is a universal method and can be applied to image complex shapes of a range of structures, like meristems, flowers, stems, leaves, or various types of trichomes. Different plant species growing in various conditions can be studied.
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References
Green PB, Erickson RO, Richmond PA (1970) On the physical basis of cell morphogenesis. Ann N Y Acad Sci 175:712–731
Hejnowicz Z, Nakielski J (1979) Modeling of growth in shoot apical dome. Acta Soc Bot Pol 48:423–442
Silk WK, Lord EM, Eckard KJ (1989) Growth patterns inferred from anatomical records: empirical tests using longisections of roots of Zea mays L. Plant Physiol 90:708–713
Hejnowicz Z, Nakielski J, Włoch W et al (1988) Growth and development of the shoot apex of barley III. Study of growth rate variation by means of the growth tensor. Acta Soc Bot Pol 57:31–50
Williams MH (1991) A sequential study of cell divisions and expansion patterns on a single developing shoot apex of Vinca major. Ann Bot 68:541–546
Williams MH, Green PB (1988) Sequential scanning electron microscopy of a growing plant meristem. Protoplasma 147:77–79
Dumais J, Kwiatkowska D (2002) Analysis of surface growth in shoot apices. Plant J 31: 229–241
Routier-Kierzkowska A-L, Kwiatkowska D (2008) New stereoscopic reconstruction protocol for scanning electron microscope images and its application to in vivo replicas of the shoot apical meristem. Funct Plant Biol 35:1034–1046
Uyttewaal M, Burian A, Alim K et al (2012) Mechanical stress acts via katanin to amplify differences in growth rate between adjacent cells in Arabidopsis. Cell 149:439–451
Green PB, Havelange A, Bernier G (1991) Floral morphogenesis in Anagallis: scanning-electron-micrograph sequences from individual growing meristems before, during, and after the transition to flowering. Planta 185: 502–512
Andréasson E, Jørgensen LB, Höglund A-S et al (2001) Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus. Plant Physiol 127:1750–1763
Bones A, Rossiter JT (1996) The myrosinase-glucosinolate system, its organization and biochemistry. Physiol Plant 97:194–208
Elsner J, Michalski M, Kwiatkowska D (2012) Spatiotemporal variation of leaf epidermal cell growth: a quantitative analysis of Arabidopsis thaliana wild-type and triple cyclinD3 mutant plants. Ann Bot 109:897–910
Kwiatkowska D, Routier-Kierzkowska A-L (2009) Morphogenesis at the inflorescence shoot apex of Anagallis arvensis: surface geometry and growth in comparison with the vegetative shoot. J Exp Bot 60:3407–3418
Kwiatkowska D (2006) Flower primordium formation at the Arabidopsis shoot apex: quantitative analysis of surface geometry and growth. J Exp Bot 57:571–580
Kwiatkowska D (2004) Surface growth at the reproductive shoot apex of Arabidopsis thaliana: pin-formed 1 and wild type. J Exp Bot 55:1021–1032
Geisler MJ, Sack FD (2002) Variable timing of developmental progression in the stomatal pathway in Arabidopsis cotyledons. New Phytol 153:469–476
Acknowledgments
The sequential replica method has been developed by the late Paul B. Green. While writing this chapter, we have used his numerous indispensable advices that we have learned from Dr. Jacques Dumais, the last graduate student of Paul. We would like to thank Drs. Zofia Czarna and Krystyna Heller (Electron Microscopy Laboratory, Wrocław University of Agricultural Sciences, Poland) and Ewa Teper (Laboratory of Scanning Electron Microscopy, Faculty of Earth Sciences, University of Silesia) for their help with scanning electron microscopy and Dr. Joanna Elsner (University of Silesia) for providing SEM micrographs of leaf epidermis. The work in D.K. lab is financially supported by Polish Ministry of Science and Higher Education and by the MAESTRO research grant No 2011/02/A/NZ3/00079 from the National Science Centre, Poland.
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Kwiatkowska, D., Burian, A. (2014). Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_8
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DOI: https://doi.org/10.1007/978-1-62703-643-6_8
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