Abstract
Optical tweezers allow noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi-)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe some working protocols to trap intracellular structures in plant cells.
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Ketelaar, T., de Ruijter, N., Niehren, S. (2014). Optical Trapping in Plant Cells. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_22
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DOI: https://doi.org/10.1007/978-1-62703-643-6_22
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-642-9
Online ISBN: 978-1-62703-643-6
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