Abstract
This protocol describes a quantitative analysis of the morphology of small plants from the moss Physcomitrella patens. The protocol can be used for the analysis of growth phenotypes produced by transient RNA interference or for the analysis of stable mutant plants. Information is presented to guide the investigator in the choice of vectors and basic conditions to perform transient RNA interference in moss. Detailed directions and examples for fluorescence image acquisition of small regenerating moss plants are provided. Instructions for the use of an ImageJ-based macro for quantitative morphological analysis of these plants are also provided.
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Acknowledgments
This work was supported by NSF (IOS 1002837 and MCB 1253444 to L.V.). We thank Erin Agar, Jennifer Garbarino, and Graham Burkart for comments on this manuscript and to Luisanna Paulino for sample images. We also thank other members of the Vidali and Bezanilla laboratories for their support.
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Bibeau, J.P., Vidali, L. (2014). Morphological Analysis of Cell Growth Mutants in Physcomitrella . In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_17
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DOI: https://doi.org/10.1007/978-1-62703-643-6_17
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-643-6
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