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Essential Methods of Plant Sample Preparation for Light Microscopy

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1080))

Abstract

There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Hereby we summarize some of the “classical” techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).

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Acknowledgment

This work has been supported by the COST-LD11017 project.

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Authors and Affiliations

  1. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic

    Aleš Soukup & Edita Tylová

Authors
  1. Aleš Soukup
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  2. Edita Tylová
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Editor information

Editors and Affiliations

  1. Laboratory of Cell Biology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Viktor Žárský

  2. Faculty of Science, Charles University, Prague, Czech Republic

    Fatima Cvrčková

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Soukup, A., Tylová, E. (2014). Essential Methods of Plant Sample Preparation for Light Microscopy. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_1

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  • DOI: https://doi.org/10.1007/978-1-62703-643-6_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-642-9

  • Online ISBN: 978-1-62703-643-6

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