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Plant Proteomics pp 111–129Cite as

Plant Proteomics Methods to Reach Low-Abundance Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1072))

Abstract

The question of low-abundance proteins from biological tissues is still a major issue. Technologies have been devised to improve the situation and in the last few years a method based on solid-phase combinatorial peptide ligand libraries has been extensively applied to animal extracts. This method has also been extended to plant extracts taking advantage of findings from previous experience. Detailed methods are described and their pertinence highlighted according to various situations of plant sample origin, size of the sample, and analytical methods intended to be used for protein identifications.

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Abbreviations

CPLL:

Combinatorial peptide ligand library

TUC:

Thiourea–urea-CHAPS

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Author information

Authors and Affiliations

  1. EB-Consult, Croissy, France

    Egisto Boschetti

  2. Miles Gloriosus Academy, Milano, Italy

    Pier Giorgio Righetti

Authors
  1. Egisto Boschetti
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  2. Pier Giorgio Righetti
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Editor information

Editors and Affiliations

  1. Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain

    Jesus V. Jorrin-Novo

  2. University of Tsukuba National Institute of Crop Science, Tsukuba, Japan

    Setsuko Komatsu

  3. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Wolfram Weckwerth

  4. Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Stefanie Wienkoop

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Boschetti, E., Righetti, P.G. (2014). Plant Proteomics Methods to Reach Low-Abundance Proteins. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_9

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  • DOI: https://doi.org/10.1007/978-1-62703-631-3_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-630-6

  • Online ISBN: 978-1-62703-631-3

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