Abstract
Substantial evidences implicate that sample preparation and protein extraction in proteomic studies of plant–pathogen interactions are critical to understand cross talk between host and pathogen. Therefore, interest is growing in applying proteomics techniques to investigate simultaneously secreted proteins from rice and pathogen. We have found, however, that most proteins of interest are low abundant so that proper prefractionation or extraction of secreted proteins from extracellular space (ECS) in the rice leaf is required to excavate relevant protein. This chapter describes the preparation of sample and extraction procedure to enrich the proteins interested before separation by 2-DE or LC-MS/MS. This method significantly increases the sensitivity of proteomic comparisons.
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Acknowledgments
This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (Ministry of Education, Science and Technology) (NRF-2011-355-C00151) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2011-0027211 and 2011-0018092).
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Wang, Y., Kim, S.G., Wu, J., Kim, S.T., Kang, K.Y. (2014). Differential Proteome and Secretome Analysis During Rice–Pathogen Interaction. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_38
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DOI: https://doi.org/10.1007/978-1-62703-631-3_38
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