Abstract
Plastids are specialized cell organelles in plant cells that are differentiated into various forms including chloroplasts, chromoplasts, and amyloplasts, and fulfill important functions in maintaining the overall cell metabolism and sensing environmental factors such as sunlight. It is therefore important to grasp the mechanisms of differentiation and functional changes of plastids in order to enhance the understanding of vegetality. In this chapter, details of a method for the extraction of intact plastids that makes analysis possible while maintaining the plastid functions are provided; in addition, a quantitative shotgun method for analyzing the composition and changes in the content of proteins in plastids as a result of environmental impacts is described.
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Acknowledgement
This research was supported by Grants-in-Aid for Scientific Research (B) (22380186) and Scientific Research on Innovative Areas (22114507) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Shiraya, T., Kaneko, K., Mitsui, T. (2014). Quantitative Proteomic Analysis of Intact Plastids. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_32
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DOI: https://doi.org/10.1007/978-1-62703-631-3_32
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