Abstract
Whole-mount in situ hybridization (WISH) is a method to visualize gene expression through hybridization of in vitro synthesized riboprobes to cellular mRNAs. WISH has been used in developmental biology for decades and was adapted to many species, especially for model organisms of developmental biology. The method has evolved, from analyzing small embryo batches to large-scale gene expression screenings, using: (1) manual or automated protocols, (2) colorimetric or fluorescent detection of the hybridized riboprobes, and (3) individual or systemic image acquisition and storage. As for bovine embryo staging, the in situ hybridization of whole embryonic discs has both proved useful and efficient, provided that a few improvements were brought to the in vitro riboprobe synthesis.
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Acknowledgments
Most of the work concerning embryo staging through whole-mount in situ hybridization was done with the support of INRA, EU and ANR grants and/or DFG grants, as specified in each of our publications, but also with the funding for French-German exchanges through DAAD and PHC (Procope). We also gratefully acknowledge the colleagues who helped us publishing nice WISH data (in Bonn: S. Molly and H. Langmann, in Jouy: E. Campion) and having access to embryos (in Jouy: J. Marchal, C. Richard).
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Hue, I., Degrelle, S.A., Viebahn, C. (2013). Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos. In: Alberio, R. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 1074. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-628-3_10
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DOI: https://doi.org/10.1007/978-1-62703-628-3_10
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