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Virus-Host Interactions pp 131–140Cite as

Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1064))

Abstract

Nucleocytoplasmic trafficking of proteins plays important roles in processes of the viral life cycle. Interspecies heterokaryon assay is one of the most effective methods to investigate the nucleocytoplasmic trafficking properties of a protein. In our lab, the interspecies heterokaryon assay has been applied to identify a few herpesviral proteins with nucleocytoplasmic shuttling property. In this chapter, the detailed information and methods of the heterokaryon assay are presented.

Shuai Wang and Kezhen Wang contributed equally to this chapter.

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Acknowledgments

This work was supported by the grants from the Start-Up Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141); the National Natural Science Foundation of China (81171584, 81101263); the Program for Changjiang Scholars and Innovative Research Team in Soochow University and the Program for Jiangsu Provincial Innovative Research Team.

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Authors and Affiliations

  1. Institute of Biology and Medical Sciences, Soochow University, Jiangsu Suzhou, P.R. China

    Shuai Wang, Kezheng Wang & Chunfu Zheng

Authors
  1. Shuai Wang
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  2. Kezheng Wang
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  3. Chunfu Zheng
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Editor information

Editors and Affiliations

  1. University of Stuttgart Institute of Interfacial Process, Stuttgart, Germany

    Susanne M. Bailer

  2. Ulm University Medical Center Institute of Virology, Ulm, Germany

    Diana Lieber

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Wang, S., Wang, K., Zheng, C. (2013). Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins. In: Bailer, S., Lieber, D. (eds) Virus-Host Interactions. Methods in Molecular Biology, vol 1064. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-601-6_9

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  • DOI: https://doi.org/10.1007/978-1-62703-601-6_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-600-9

  • Online ISBN: 978-1-62703-601-6

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