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Detection of Integrated Herpesvirus Genomes by Fluorescence In Situ Hybridization (FISH)

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Virus-Host Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1064))

Abstract

Fluorescence in situ hybridization (FISH) is widely used to visualize nucleotide sequences in interphase cells or on metaphase chromosomes using specific probes that are complementary to the respective targets. Besides its broad application in cytogenetics and cancer research, FISH facilitates the localization of virus genomes in infected cells. Some herpesviruses, including human herpesvirus 6 (HHV-6) and Marek’s disease virus (MDV), have been shown to integrate their genetic material into host chromosomes, which allows transmission of HHV-6 via the germ line and is required for efficient MDV-induced tumor formation. We describe here the detection by FISH of integrated herpesvirus genomes in metaphase chromosomes and interphase nuclei of herpesvirus-infected cells.

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Acknowledgments

The author thanks Dr. Nikolaus Osterrieder for editing the manuscript. This work was supported by the DFG grant KA3492.1-1 and funding from the Freie Universität Berlin to B.B.K.

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Authors and Affiliations

  1. Institute of Virology, Freie Universität Berlin, Berlin, Germany

    Benedikt B. Kaufer

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  1. Benedikt B. Kaufer
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Editors and Affiliations

  1. University of Stuttgart Institute of Interfacial Process, Stuttgart, Germany

    Susanne M. Bailer

  2. Ulm University Medical Center Institute of Virology, Ulm, Germany

    Diana Lieber

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Kaufer, B.B. (2013). Detection of Integrated Herpesvirus Genomes by Fluorescence In Situ Hybridization (FISH). In: Bailer, S., Lieber, D. (eds) Virus-Host Interactions. Methods in Molecular Biology, vol 1064. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-601-6_10

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  • DOI: https://doi.org/10.1007/978-1-62703-601-6_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-600-9

  • Online ISBN: 978-1-62703-601-6

  • eBook Packages: Springer Protocols

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