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Using a Reverse Genetics Approach to Investigate Small-Molecule Activity

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Plant Chemical Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1056))

Abstract

Chemical genomics is a highly effective approach for understanding complex and dynamic biological processes in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a huge abundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here we present an approach to characterize a chemical of interest, as well as examples of studies demonstrating an effective combination of chemical genomics with reverse genetics strategies, drawn from recent literature on phytohormone signalling and auxin transport.

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Acknowledgements

We thank the Vetenskapsrådet and VINNOVA for supporting this work.

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Authors and Affiliations

  1. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences (SLU), Umeå, Sweden

    Siamsa M. Doyle & Stéphanie Robert

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  1. Siamsa M. Doyle
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  2. Stéphanie Robert
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Editor information

Editors and Affiliations

  1. Department of Botany & Plant Sciences, University of California, Riverside, Riverside, USA

    Glenn R Hicks

  2. Department of Forest Genetics and Plant Umeå Plant Science Centre, Swedish University of Agricultural Scien, Umeå, Sweden

    Stéphanie Robert

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Doyle, S.M., Robert, S. (2014). Using a Reverse Genetics Approach to Investigate Small-Molecule Activity. In: Hicks, G., Robert, S. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 1056. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-592-7_6

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  • DOI: https://doi.org/10.1007/978-1-62703-592-7_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-591-0

  • Online ISBN: 978-1-62703-592-7

  • eBook Packages: Springer Protocols

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