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Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies

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Immunoproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1061))

Abstract

Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients’ antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.

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Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, University of Copenhagen, København, Denmark

    Alexander Nøstdal & Hans H. Wandall

Authors
  1. Alexander Nøstdal
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  2. Hans H. Wandall
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Editor information

Editors and Affiliations

  1. National Research Council Canada Human Health Therapeutics, Ottawa, Ontario, Canada

    Kelly M. Fulton

  2. National Research Council Canada Human Health Therapeutics, Ottawa, Ontario, Canada

    Susan M. Twine

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Nøstdal, A., Wandall, H.H. (2013). Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies. In: Fulton, K., Twine, S. (eds) Immunoproteomics. Methods in Molecular Biology, vol 1061. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-589-7_10

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  • DOI: https://doi.org/10.1007/978-1-62703-589-7_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-588-0

  • Online ISBN: 978-1-62703-589-7

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