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Human Monoclonal Antibodies pp 309–330Cite as

Methods for Radiolabelling of Monoclonal Antibodies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1060))

Abstract

The use of radionuclide labels allows to study the pharmacokinetics of monoclonal antibodies, to control the specificity of their targeting and to monitor the response to an antibody treatment with high accuracy. Selection of label depends on the processing of an antibody after binding to an antigen, and on the character of information to be derived from the study (distribution of antibody in the extracellular space, target occupancy or determination of sites of metabolism). This chapter provides protocols for labelling of antibodies with iodine-125 (suitable also for other radioisotopes of iodine) and with indium-111. Since radiolabelling might damage and reduce the immunoreactive fraction and/or affinity of an antibody, the methods for assessment of these characteristics of an antibody are provided for control.

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Author information

Authors and Affiliations

  1. Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden

    Vladimir Tolmachev

  2. Preclinical PET Platform, Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden

    Anna Orlova

  3. Unit of Biomedical Radiation Sciences and Rudbeck Laboratory, Ridgeview Instruments AB, Uppsala, Sweden

    Karl Andersson

Authors
  1. Vladimir Tolmachev
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  2. Anna Orlova
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  3. Karl Andersson
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Editor information

Editors and Affiliations

  1. Department of Pathology, The Lautenberg Center, IMRIC The Hebrew University Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

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Tolmachev, V., Orlova, A., Andersson, K. (2014). Methods for Radiolabelling of Monoclonal Antibodies. In: Steinitz, M. (eds) Human Monoclonal Antibodies. Methods in Molecular Biology, vol 1060. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-62703-586-6_16

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  • DOI: https://doi.org/10.1007/978-1-62703-586-6_16

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-62703-585-9

  • Online ISBN: 978-1-62703-586-6

  • eBook Packages: Springer Protocols

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