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Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy

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Metabolomics Tools for Natural Product Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1055))

Abstract

NMR-based biochemical profiling of natural products has become popular due to the development of high-resolution instruments (>400 MHz) and cryogenically cooled probes/preamplifiers, by increasing the sensitivity of NMR instruments several fold and reducing instrument noise levels. NMR provides a rapid, nondestructive, high-throughput method that requires minimal sample preparation, therefore maintaining the biological integrity of the sample. One-dimensional (1D) solution-state 1H NMR is used in untargeted sample screening (metabolomics/metabonomics) to gain insight into spectral pattern changes associated with samples of different origins. Metabolomics and metabonomics contextually explains the systematic and quantitative measurement of metabolites that are produced from the biochemical reactions of living systems. This chapter describes some commonly used 1H NMR experiments for identification and quantification of small molecular weight, water soluble metabolites in biological samples, some considerations for choosing the correct NMR experiment, and sample preparation protocols for isolating metabolites from a number of biological sample types.

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Acknowledgments

Dr John R. Sheedy would like to thank Dr. Berin Boughton from Metabolomics Australia (The University of Melbourne) and Dr Sara Long from CAPIM (The University of Melbourne) for their contributions to the biological tissue homogenization work. I would also like to thank Professor David Gardner and Dr. George Thouas (Zoology, The University of Melbourne) for providing embryo culture media samples to evaluate using NMR for profiling and quantifying media components.

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Authors and Affiliations

  1. Department of Zoology, The University of Melbourne, Parkville, VIC, Australia

    John Robert Sheedy

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  1. John Robert Sheedy
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Editor information

Editors and Affiliations

  1. Parkville, Australia

    Ute Roessner

  2. School of Botany, The University of Melbourne, Parkville, Victoria, Australia

    Daniel Anthony Dias

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Sheedy, J.R. (2013). Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy. In: Roessner, U., Dias, D. (eds) Metabolomics Tools for Natural Product Discovery. Methods in Molecular Biology, vol 1055. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-577-4_7

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  • DOI: https://doi.org/10.1007/978-1-62703-577-4_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-576-7

  • Online ISBN: 978-1-62703-577-4

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