Abstract
NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of “clinical grade” human neural stem cell lines.
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References
Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255(5052):1707–1710
Aboody K et al (2011) Translating stem cell studies to the clinic for CNS repair: current state of the art and the need for a Rosetta Stone. Neuron 70(4):597–613
de Filippis L (2011) Neural stem cell-mediated therapy for rare brain diseases: perspectives in the near future for LSDs and MNDs. Histol Histopathol 26(8):1093–1109
Kim SU, de Vellis J (2009) Stem cell-based cell therapy in neurological diseases: a review. J Neurosci Res 87(10):2183–2200
Lindvall O, Kokaia Z (2010) Stem cells in human neurodegenerative disorders–time for clinical translation? J Clin Invest 120(1):29–40
EU GMP. The rules governing medicinal products in the European Union. Volume 4 EU guidelines to good manufacturing practice—medicinal products for human and veterinary use-, Annex 1–2008
European Pharmacopeia 7th edn. volume 2, Chapter 2.6 Biological tests–2010
Galli R, Gritti A, Vescovi AL (2008) Adult neural stem cells. Methods Mol Biol 438:67–84
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Gelati, M., Profico, D., Projetti-Pensi, M., Muzi, G., Sgaravizzi, G., Vescovi, A.L. (2013). Culturing and Expansion of “Clinical Grade” Precursors Cells from the Fetal Human Central Nervous System. In: Reynolds, B., Deleyrolle, L. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 1059. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-574-3_6
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DOI: https://doi.org/10.1007/978-1-62703-574-3_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-573-6
Online ISBN: 978-1-62703-574-3
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