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Regulation of the Mutator System of Transposons in Maize

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Plant Transposable Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1057))

Abstract

The Mutator system has proved to be an invaluable tool for elucidating gene function via insertional mutagenesis. Its high copy number, high transposition frequency, relative lack of insertion specificity, and ease of use has made it the preferred method for gene tagging in maize. Recent advances in high throughput sequencing of insertion sites, combined with the availability of large numbers of pre-mutagenized and sequence-indexed stocks, ensure that this resource will only be more useful in the years ahead. Muk is a locus that can silence Mu-active lines, making it possible to ameliorate the phenotypic effects of high numbers of active Mu transposons and reduce the copy number of these elements during introgressions.

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Author information

Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA

    Damon Lisch

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  1. Damon Lisch
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Editors and Affiliations

  1. Dept. Genetics, Development & Cell Biology, Iowa State University, Ames, Iowa, USA

    Thomas Peterson

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Lisch, D. (2013). Regulation of the Mutator System of Transposons in Maize. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_9

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  • DOI: https://doi.org/10.1007/978-1-62703-568-2_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-567-5

  • Online ISBN: 978-1-62703-568-2

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