Abstract
To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile Ac element driven by the CaMV 35S promoter, and a gene trap Ds carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a Ds transposant population was achieved using a plant regeneration procedure involving the tissue culture of seed-derived calli carrying Ac and Ds elements. During tissue cultures, Ds mobility accompanies changes in methylation patterns of a terminal region of Ds, where over 70 % of plants contained independent Ds insertions. In the transposon population, around 12 % of plants expressed GUS at the early seedling stage. A flanking-sequence-tag (FST) database has been established by cloning over 19,968 Ds insertion sites and the Ds map shows relatively uniform distribution across the rice chromosomes.
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Acknowledgement
This research was supported by grants from the Next-Generation BioGreen 21 Program (PJ008215 and PJ008168), Rural Development Administration, Republic of Korea. Y.H.X. was supported by a scholarship from the BK21 program.
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Xuan, Y.H. et al. (2013). Plant Regeneration Methods for Rapid Generation of a Large Scale Ds Transposant Population in Rice. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_7
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DOI: https://doi.org/10.1007/978-1-62703-568-2_7
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