Skip to main content

Using MuDR/Mu Transposons in Directed Tagging Strategies

  • Protocol
  • First Online:
Plant Transposable Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1057))

Abstract

An introduction to MuDR/Mu transposons as mutagens is provided along with protocols for using these elements to tag maize genes. Selection for retention of Mutator activity is described as well as details for establishing and screening tagging populations efficiently.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Walbot V, Rudenko GN (2002) MuDR/Mu transposons of maize. In: Craig NL, Craigie R, Gellert M, Lambowitz A (eds) Mobile DNA II. American Society of Microbiology, Washington, DC, pp 533–564

    Google Scholar 

  2. Athma P, Grotewold E, Peterson T (1992) Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics 131:199–209

    PubMed  CAS  Google Scholar 

  3. Lisch D (2002) Mutator transposons. Trends Plant Sci 7:498–504

    Article  PubMed  CAS  Google Scholar 

  4. Lisch D, Jiang N (2009) Mutator and MULE transposons. In: Bennetzen JL, Hake S (eds) Handbook of maize genetics and genomics. Springer, New York, pp 277–306

    Chapter  Google Scholar 

  5. Walbot V (1986) Inheritance of mutator activity in Zea mays as assayed by somatic instability of the bz2-mu1 allele. Genetics 114:1293–1312

    PubMed  CAS  Google Scholar 

  6. McLaughlin M, Walbot V (1987) Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization. Genetics 117:771–776

    PubMed  CAS  Google Scholar 

  7. Robertson DS (1986) Genetic studies on the loss of Mu mutator activity in maize. Genetics 113:765–773

    PubMed  CAS  Google Scholar 

  8. Levy AA, Walbot V (1990) Regulation of the timing of transposable element excision during maize development. Science 248:1534–1537

    Article  PubMed  CAS  Google Scholar 

  9. Rudenko GN, Walbot V (2001) Expression and post-transcriptional regulation of maize transposable element MuDR and its derivatives. Plant Cell 13:553–570

    PubMed  CAS  Google Scholar 

  10. Fernandes J et al (2004) Genome-wide mutagenesis of Zea mays L. using RescueMu transposons. Genome Biol 5:82

    Article  Google Scholar 

  11. Slotkin RK, Freeling M, Lisch D (2003) Mu killer causes the heritable inactivation of the mutator family of transposable elements in Zea mays. Genetics 165:781–797

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Research support in the Walbot laboratory is provided from a grant from the National Science Foundation (PGRP 07–01880). J.Q. was supported by a Fulbright award for a 3 month visit to Stanford University during her Ph.D. program.

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Walbot, V., Qüesta, J. (2013). Using MuDR/Mu Transposons in Directed Tagging Strategies. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-568-2_10

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-567-5

  • Online ISBN: 978-1-62703-568-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics