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Cell Sorting of Young and Senescent Cells

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Biological Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1048))

Abstract

Cellular senescence is the irreversible loss of proliferative potential and is accompanied by a number of phenotypic changes. First described by Hayflick and Moorhead in 1961, it has since become a popular model to study cellular aging. The replicative lifespan of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing with each population doubling (PD). Thus, the study of individual cells in mass culture is necessary in order to properly understand senescence and its associated phenotype. Cell sorting is a process that allows the physical separation of cells based on different characteristics which can be measured by flow cytometry. Here, we describe various methods by which senescent cells can be sorted from mixed cultures and discuss how different methods impact on the posterior analysis of sorted populations.

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Acknowledgment

This work was supported by a BBSRC David Phillips Fellowship awarded to J.P.

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Authors and Affiliations

  1. Ageing Research Laboratories, Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle, UK

    Graeme Hewitt, Thomas von Zglinicki & João F. Passos

Authors
  1. Graeme Hewitt
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  2. Thomas von Zglinicki
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  3. João F. Passos
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Editor information

Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, Alabama, USA

    Trygve O. Tollefsbol

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Hewitt, G., von Zglinicki, T., Passos, J.F. (2013). Cell Sorting of Young and Senescent Cells. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_4

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  • DOI: https://doi.org/10.1007/978-1-62703-556-9_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-555-2

  • Online ISBN: 978-1-62703-556-9

  • eBook Packages: Springer Protocols

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