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Peptide Nucleic Acids pp 1–12Cite as

MiniPEG-γPNA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1050))

Abstract

Peptide nucleic acids (PNAs) are attractive, as compared to other classes of oligonucleotides that have been developed to date, in that they are relatively easy to synthesize and modify, hybridize to DNA and RNA with high affinity and sequence selectivity, and are resistant to enzymatic degradation by proteases and nucleases; however, the downside is that they are only moderately soluble in aqueous solution. Herein we describe the protocols for synthesizing the second-generation γPNAs, both the monomers and oligomers, containing MiniPEG side chain with considerable improvements in water solubility, biocompatibility, and hybridization properties.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Carnegie Mellon University, Pittsburgh, USA

    Arunava Manna, Srinivas Rapireddy, Raman Bahal & Danith H. Ly

Authors
  1. Arunava Manna
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  2. Srinivas Rapireddy
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  3. Raman Bahal
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  4. Danith H. Ly
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Editor information

Editors and Affiliations

  1. Dept. Cellular & Molecular Medicine, University of Copenhagen Faculty of Health Sciences, Copenhagen N, Denmark

    Peter E Nielsen

  2. NIH NIDDK, Bethesda, USA

    Daniel H. Appella

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Manna, A., Rapireddy, S., Bahal, R., Ly, D.H. (2014). MiniPEG-γPNA. In: Nielsen, P., Appella, D. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 1050. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-553-8_1

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  • DOI: https://doi.org/10.1007/978-1-62703-553-8_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-552-1

  • Online ISBN: 978-1-62703-553-8

  • eBook Packages: Springer Protocols

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