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New Opportunities for Immobilization of Enzymes

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1051))

Abstract

In this chapter, as a general introduction, we summarize our personal point of view on immobilization technique in order to prepare optimal and cost-effective biocatalysts. Special attention is paid to the improvement of enzyme properties via immobilization techniques. From the stabilization by multipoint covalent attachment to the generation of hydrophilic environments via post-immobilization techniques are here discussed. Immobilization techniques, a necessary tool to reuse enzyme, have to be simple and, if possible, may become a very powerful tool to greatly improve properties of every kind of enzymes: monomeric, multimeric, stable, labile, poorly selective, etc.

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Authors and Affiliations

  1. Instituto de Catalisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

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  1. Jose M. Guisan
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  1. Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

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Guisan, J.M. (2013). New Opportunities for Immobilization of Enzymes. In: Guisan, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 1051. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-550-7_1

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  • DOI: https://doi.org/10.1007/978-1-62703-550-7_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-549-1

  • Online ISBN: 978-1-62703-550-7

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