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Nucleic Acid Detection pp 169–179Cite as

Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1039))

Abstract

Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3 2+-conjugated silica nanoparticles (RuSi@Ru(bpy)3 2+) tag-based signal amplification.

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Acknowledgment

This work was supported by the 973 Program (Grant 2012CB932600), the National Natural Science Foundation (Grants 21025522 and 20890020) of China.

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Authors and Affiliations

  1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China

    Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu & Hong-Yuan Chen

Authors
  1. Mei-Sheng Wu
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  2. Guang-sheng Qian
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  3. Jing-Juan Xu
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  4. Hong-Yuan Chen
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Editor information

Editors and Affiliations

  1. University of Central Florida Department of Chemistry, Orlando, Florida, USA

    Dmitry M. Kolpashchikov

  2. University of Central Florida Department of Chemistry, Orlando, Florida, USA

    Yulia V. Gerasimova

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Wu, MS., Qian, Gs., Xu, JJ., Chen, HY. (2013). Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode. In: Kolpashchikov, D., Gerasimova, Y. (eds) Nucleic Acid Detection. Methods in Molecular Biology, vol 1039. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-535-4_15

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  • DOI: https://doi.org/10.1007/978-1-62703-535-4_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-534-7

  • Online ISBN: 978-1-62703-535-4

  • eBook Packages: Springer Protocols

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