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Chromosomal Aberration Test in Human Lymphocytes

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Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1044))

Abstract

Human peripheral lymphocytes (HPL) are non-cycling primary cells (G0 cells). They are easily collectable by venipuncture. In the presence of suitable culture media and stimulants in vitro HPL enter the cell cycle and divide mitotically. Metaphase-like stages can be arrested using the spindle fiber poison colcemid and prepared on microscopic slides. Following appropriate staining, chromosomal aberrations can be analyzed in the microscope. These aberrations may be induced either in vivo by environmental or occupational influences or in vitro after experimentally controlled manipulations in order to detect or to test the mutagenic potency of various agents.

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Authors and Affiliations

  1. Faculty of Biology — Genetics, Center for Medical Biotechnology, University of Duisburg-Essen, Essen, Germany

    Christian Johannes & Guenter Obe

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  1. Christian Johannes
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  2. Guenter Obe
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Editors and Affiliations

  1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Alok Dhawan

  2. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Mahima Bajpayee

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Johannes, C., Obe, G. (2013). Chromosomal Aberration Test in Human Lymphocytes. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 1044. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-529-3_8

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  • DOI: https://doi.org/10.1007/978-1-62703-529-3_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-528-6

  • Online ISBN: 978-1-62703-529-3

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