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Genotoxicity Assessment pp 417–427Cite as

The Wing-Spot and the Comet Tests as Useful Assays Detecting Genotoxicity in Drosophila

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1044))

Abstract

In spite of its pioneer use in detecting mutational processes, Drosophila has yet an important role in studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer and no assays exist to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila.

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Acknowledgements

ER Carmona thanks the support given by FONDECYT (Project No. 11110181) and the Dirección General de Investigación y Postgrado, Universidad Católica de Temuco (Projects No. CD2010 DGIPUCT-01 and 0804 MECESUP UCT).

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Authors and Affiliations

  1. Grup de Mutagènesi, Departament de Genética i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Barcelona, Spain

    Ricard Marcos

  2. Escuela de Ciencias Ambientales, Núcleo de Investigación en Estudios Ambientales, Universidad Católica de Temuco, Temuco, Chile

    Erico R. Carmona

Authors
  1. Ricard Marcos
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  2. Erico R. Carmona
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Editors and Affiliations

  1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Alok Dhawan

  2. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Mahima Bajpayee

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Marcos, R., Carmona, E.R. (2013). The Wing-Spot and the Comet Tests as Useful Assays Detecting Genotoxicity in Drosophila. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 1044. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-529-3_23

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  • DOI: https://doi.org/10.1007/978-1-62703-529-3_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-528-6

  • Online ISBN: 978-1-62703-529-3

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