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Isochronal Visualization of Transcription and Proteasomal Proteolysis in Cell Culture or in the Model Organism, Caenorhabditis elegans

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Imaging Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1042))

Abstract

Investigation of differential gene regulation by protein degradation requires analysis of the spatial and temporal association between proteolysis and transcription. Here, we describe the isochronal visualization of proteasomal proteolysis and transcription in cell culture or in vivo in the model organism Caenorhabditis elegans. This includes localization of proteasome-dependent proteolysis by fluorescent degradation products of model and endogenous substrates of the proteasome in combination with immunolabelling of RNA polymerase II and transcription in situ run-on assays.

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Acknowledgments

Work in the von Mikecz laboratory is supported by the German Science Foundation (DFG) through grants MI486/7-1 and GRK1033.

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Authors and Affiliations

  1. IUF – Leibniz Research Institute for Environmental Medicine at Heinrich-Heine University Düsseldorf, Düsseldorf, Germany

    Anna von Mikecz & Andrea Scharf

Authors
  1. Anna von Mikecz
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  2. Andrea Scharf
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Editors and Affiliations

  1. Bar-Ilan University Inst. of Nanotech & Advanced Materials, Ramat Gan, Israel

    Yaron Shav-Tal

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von Mikecz, A., Scharf, A. (2013). Isochronal Visualization of Transcription and Proteasomal Proteolysis in Cell Culture or in the Model Organism, Caenorhabditis elegans . In: Shav-Tal, Y. (eds) Imaging Gene Expression. Methods in Molecular Biology, vol 1042. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-526-2_19

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  • DOI: https://doi.org/10.1007/978-1-62703-526-2_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-525-5

  • Online ISBN: 978-1-62703-526-2

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