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The Dynamics of DNA Damage Repair and Transcription

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Book cover Imaging Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1042))

Abstract

Recent advances have led to several systems to study transcription from defined loci in living cells. It has now become possible to address long-standing questions regarding the interplay between the processes of DNA damage repair and transcription—two disparate processes that can occur on the same stretch of chromatin and which both lead to extensive chromatin change. Here we describe the development of a system to create enzymatically induced DNA double-strand breaks (DSBs) at a site of inducible transcription and methods to study the interplay between these processes.

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Authors and Affiliations

  1. Department of Cancer Biology, Abramson Family Cancer Research Institute, Basser Research Center for BRCA1/2, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Niraj M. Shanbhag & Roger A. Greenberg

  2. Department of Pathology, Abramson Family Cancer Research Institute, Basser Research Center for BRCA1/2, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Roger A. Greenberg

Authors
  1. Niraj M. Shanbhag
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  2. Roger A. Greenberg
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Corresponding author

Correspondence to Roger A. Greenberg .

Editor information

Editors and Affiliations

  1. Bar-Ilan University Inst. of Nanotech & Advanced Materials, Ramat Gan, Israel

    Yaron Shav-Tal

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Shanbhag, N.M., Greenberg, R.A. (2013). The Dynamics of DNA Damage Repair and Transcription. In: Shav-Tal, Y. (eds) Imaging Gene Expression. Methods in Molecular Biology, vol 1042. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-526-2_16

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  • DOI: https://doi.org/10.1007/978-1-62703-526-2_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-525-5

  • Online ISBN: 978-1-62703-526-2

  • eBook Packages: Springer Protocols

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