Abstract
The appropriate functioning of living cells depends on a variety of dynamic processes that necessitate delicate motion, transportation, association, and disassociation in time and space. Different dynamic patterns such as directed motion, normal diffusion, and restricted diffusion take part at different length scales, and their identification serves as a tool for exploring biochemical processes. Here we describe single-particle tracking which is a powerful method that allows the characterization of dynamic processes on the single-molecule or single-particle level with nanometer spatial and sub-second temporal precision. In particular, we describe the cell preparation procedures, microscopy imaging, and image analysis processes for following telomere dynamics in living mammalian cells.
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Acknowledgements
This work was partially supported by the Israel Science Foundation grants No. 985/08, 1729/08, 1793/07, and 507/07 and the Wolfson Foundation grant 2008.
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Berger, I.B., Kepten, E., Garini, Y. (2013). Single-Particle Tracking for Studying the Dynamic Properties of Genomic Regions in Live Cells. In: Shav-Tal, Y. (eds) Imaging Gene Expression. Methods in Molecular Biology, vol 1042. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-526-2_10
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DOI: https://doi.org/10.1007/978-1-62703-526-2_10
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