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Microglia pp 71–82Cite as

Microglial Activation: Measurement of Cytokines by Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1041))

Abstract

Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen–antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.

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Author information

Authors and Affiliations

  1. Department of cellular and Molecular Neurosciences, National Brain Research Centre, Manesar, India

    Deepak Kumar Kaushik

  2. National Brain Research Centre, Manesar, India

    Anirban Basu

Authors
  1. Deepak Kumar Kaushik
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  2. Anirban Basu
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Editor information

Editors and Affiliations

  1. , Department of Oncology-Pathology, Karolinska Institutet, Solnavägen, Stockholm, SE-171 77, Sweden

    Bertrand Joseph

  2. , Department of Biochemistry, University of Seville, García González 2, Seville, 41012, Spain

    José Luis Venero

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© 2013 Springer Science+Business Media New York

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Kaushik, D.K., Basu, A. (2013). Microglial Activation: Measurement of Cytokines by Flow Cytometry. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_9

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  • DOI: https://doi.org/10.1007/978-1-62703-520-0_9

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-519-4

  • Online ISBN: 978-1-62703-520-0

  • eBook Packages: Springer Protocols

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