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Microglia pp 63–67Cite as

Lentiviral Transduction of Cultured Microglia

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1041))

Abstract

Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past several years. Here, we describe lentiviral-mediated transduction as a valuable tool for transduction of cultured microglial cells.

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Acknowledgment

Dr. Hiroyuki Miyoshi (RIKEN BioResource Center) kindly provided the lentiviral vector and its packaging plasmids.

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Authors and Affiliations

  1. Kyushu University, Fukuoda, Japan

    Takahiro Masuda, Makoto Tsuda & Hidetoshi Tozaki-Saitoh

  2. Kyushu University, Fukuoka, Japan

    Kazuhide Inoue

Authors
  1. Takahiro Masuda
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  2. Makoto Tsuda
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  3. Hidetoshi Tozaki-Saitoh
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  4. Kazuhide Inoue
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Editor information

Editors and Affiliations

  1. , Department of Oncology-Pathology, Karolinska Institutet, Solnavägen, Stockholm, SE-171 77, Sweden

    Bertrand Joseph

  2. , Department of Biochemistry, University of Seville, García González 2, Seville, 41012, Spain

    José Luis Venero

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© 2013 Springer Science+Business Media New York

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Masuda, T., Tsuda, M., Tozaki-Saitoh, H., Inoue, K. (2013). Lentiviral Transduction of Cultured Microglia. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_8

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  • DOI: https://doi.org/10.1007/978-1-62703-520-0_8

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-519-4

  • Online ISBN: 978-1-62703-520-0

  • eBook Packages: Springer Protocols

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