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Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species

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Microglia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1041))

Abstract

Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

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Abbreviations

iNOS:

Inducible NOS

LPS:

Lipopolysaccharide

LTA:

Lipoteichoic acid

nNOS:

Neuronal NOS

NO:

Nitric oxide

NOS:

Nitric oxide synthase

PHOX:

Phagocytic NADPH oxidase

RNS:

Reactive nitrogen species

RONS:

Reactive oxygen and nitrogen species

ROS:

Reactive oxygen species

References

  1. Wyss-Coray T (2006) Inflammation in Alzheimer disease: driving force, bystander or beneficial response? Nat Med 12:1005–1015

    PubMed  CAS  Google Scholar 

  2. Ransohoff RM, Perry VH (2009) Microglial physiology: unique stimuli, specialized responses. Annu Rev Immunol 27:119–145

    Article  PubMed  CAS  Google Scholar 

  3. Colton CA, Gilbert DL (1987) Production of superoxide anions by a CNS macrophage, the microglia. FEBS Lett 223:284–288

    Article  PubMed  CAS  Google Scholar 

  4. Jekabsone A, Mander P, Tickler A et al (2006) Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study. J Neuroinflammation 3:24

    Article  PubMed  Google Scholar 

  5. Bal-Price A, Matthias A, Brown GC (2002) Stimulation of the NADPH oxidase in activated rat microglia removes nitric oxide but induces peroxynitrite production. J Neurochem 80:73–80

    Article  PubMed  CAS  Google Scholar 

  6. Mander P, Brown GC (2005) Activation of microglial NADPH oxidase is synergistic with glial iNOS expression in inducing neuronal death: a dual-key mechanism of inflammatory neurodegeneration. J Neuroinflammation 2:20

    Article  PubMed  Google Scholar 

  7. Jekabsone A, Neher J, Borutaite V et al (2007) Nitric oxide from neuronal nitric oxide synthase sensitises neurons to hypoxia-induced death via competitive inhibition of cytochrome oxidase. J Neurochem 103:346–356

    PubMed  CAS  Google Scholar 

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Acknowledgement

Relevant research in our laboratory has been funded by the Wellcome Trust (Grant RG50995).

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Authors and Affiliations

  1. University of Cambridge, Cambridge, UK

    Urte Neniskyte & Guy C. Brown

Authors
  1. Urte Neniskyte
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  2. Guy C. Brown
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Editor information

Editors and Affiliations

  1. , Department of Oncology-Pathology, Karolinska Institutet, Solnavägen, Stockholm, SE-171 77, Sweden

    Bertrand Joseph

  2. , Department of Biochemistry, University of Seville, García González 2, Seville, 41012, Spain

    José Luis Venero

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© 2013 Springer Science+Business Media New York

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Neniskyte, U., Brown, G.C. (2013). Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_12

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  • DOI: https://doi.org/10.1007/978-1-62703-520-0_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-519-4

  • Online ISBN: 978-1-62703-520-0

  • eBook Packages: Springer Protocols

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