Abstract
Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.
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Acknowledgments
This work was partially supported by a grant from the United States–Israel Binational Science Foundation (grant number 2009/290), Jerusalem, Israel, to EE. SA was supported by a Clore Fellowship.
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Alon, S., Eisenberg, E. (2013). Identifying RNA Editing Sites in miRNAs by Deep Sequencing. In: Shomron, N. (eds) Deep Sequencing Data Analysis. Methods in Molecular Biology, vol 1038. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-514-9_9
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DOI: https://doi.org/10.1007/978-1-62703-514-9_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-513-2
Online ISBN: 978-1-62703-514-9
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