Abstract
Extracellular matrix can regulate multipotent mesenchymal stromal cells (MMSC) differentiation, with potential applications for tissue engineering. A relief of mineralized bone takes essential effect on MMSC fate. Nevertheless, delicate structure and a hierarchy of niches for stromal stem cells and its quantitative parameters are not practically known. Here, we describe the protocol to define the basic approach providing a guiding for in vitro identification of quantitative features of artificial calcium phosphate niches which promotes osteogenic differentiation and maturation of stromal stem cell.
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References
Schofield R (1978) The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Blood Cells 4:7–25
Li L, Xie T (2005) Stem cell niche: structure and function. Annu Rev Cell Dev Biol 21:605–631
Dellatore SM, Garsia AS, Miller WM (2008) Mimicking stem cell niches to increase stem cell expansion. Curr Opin Biotechnol 19:534–540
Calvi LM, Adams GB, Weibrect KW et al (2003) Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 425:841–846
Yin T, Li L (2006) The stem cell niches in bone. JCI 116:1195–1201
Jing D, Fonseca A-V, Alakel N et al (2010) Hematopoietic stem cells in co-culture with mesenchymal stromal cells—modeling the niche compartments in vitro. Haematologica 95:542–550
Curtis AS, Varde M (1964) Control of cell behavior: topological factors. J Natl Cancer Inst 33:15–26
Datta N, Holtorf HL, Sikavitsas VI et al (2005) Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells. Biomaterials 26:971–977
Kolf CM, Cho E, Tuan RS (2007) Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation. Arthritis Res Ther 9:204–219
Sniadecki NJ, Desai RA, Ruiz SA, Chen CS (2006) Nanotechnology for cell-substrate interactions. Ann Biomed Eng 34:59–74
Lutolf MP, Gilbert PM, Blau HM (2009) Designing materials to direct stem-cell fate. Nature 462:433–441
Lutolf MP, Doyonnas R, Havenstrite K et al (2009) Perturbation of single hematopoietic stem cell fates in artificial niches. Integr Biol (Camb) 1:59–69
Khlusov IA, Karlov AV, Sharkeev Yu P et al (2005) Osteogenic potential of mesenchymal stem cells from bone marrow in situ: role of physicochemical properties of artificial surfaces. Bull Exp Biol Med 140:144–152
Khlusov IA, Khlusova M Yu, Zaitsev KV et al (2011) Pilot in vitro study of the parameters of artificial niche for osteogenic differentiation of human stromal stem cell pool. Bull Exp Biol Med 150:535–542
Burtis CA, Ashwood ER (eds) (2001) Tietz fundamentals of clinical chemistry, 5th edn. W.B. Saunders Company, Philadelphia
Sharkeev Yu P, Legostaeva EV, Eroshenko A Yu et al (2009) The structure and physical and mechanical properties of a novel biocomposite material, nanostructured titanium-calcium-phosphate coating. Compos Interfac 16:535–546
Aerts F, Wagemaker G (2006) Mesenchymal stem cell engineering and transplantation. In: Nolta JA (ed) Genetic engineering of mesenchymal stem cells. Springer, Dordrecht, pp 1–44
Lama VN, Smith L, Badri L et al (2007) Evidence for tissue-resident mesenchymal stem cells in human adult lung from studies of transplanted allografts. JCI 117:989–996
da Silva Meirelles L, Chagastelles PC, Nardi NB (2006) Mesenchymal stem cells reside in virtually all post-natal organs and tissues. JCS 119:2204–2213
de Bruijn JD, van den Brink I, Mendes S et al (1999) Bone induction by implants coated with cultured osteogenic bone marrow cells. Adv Dent Res 13:74–81
Riggs BL, Melton LJ III (1996) Osteoporosis. Etiology, diagnosis, and management, 2nd edn. Lippincott-Raven, Philadelphia, New York
Scadden DT (2007) The stem cell niche in health and leukemic disease. Best Pract Res Clin Haematol 20:19–27
Khlusov IA, Dekhtyar Yu, Khlusova M Yu et al (2013) Novel concepts of “Niche-Relief” and “Niche-Voltage” for stem cells as a base of bone and hematopoietic tissues biomimetic engineering. IFMBE Proc 38:99–102. doi:10.1007/978-3-642-34197-7_26
Acknowledgments
The authors are deeply indebted to: professor Yu.P. Sharkeev and E.V. Legostaeva Ph.D. (Institute of Strength Physics and Materials Science, SB of RAS, Tomsk, Russia) for designing and digital imaging of titanium specimens with calcium phosphate coating; K.V. Zaitsev Ph.D. (Stem Cells Bank Ltd., Tomsk, Russia) for cell culture provision; Joint Use Center for Materials Science (Tomsk State University, Tomsk, Russia) for microscopic equipment use.
This work was supported by the Federal Goal Program of Russian Federation (grant No 8036).
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Khlusov, I.A., Shevtsova, N.M., Khlusova, M.Y. (2013). Detection In Vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogenic Differentiation and Maturation of Stromal Stem Cells. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 1035. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-508-8_9
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DOI: https://doi.org/10.1007/978-1-62703-508-8_9
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