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Examining the Role of Mast Cells in Fetal Wound Healing Using Cultured Cells In Vitro

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Wound Regeneration and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1037))

Abstract

Mast cells play an important role during the inflammatory phase of wound healing, and studies suggest that they also influence scar formation and remodeling. Recently, our laboratory has characterized the mast cell response to injury in a fetal wound healing model. In this model, early gestation fetal skin regenerates and heals without a scar (scarless wounds) and late gestation skin heals with a scar (fibrotic wounds). Differences in mast cell number, maturity, and activity were identified between scarless and scar-forming fetal wounds. To study mast cell function in more detail, in vitro experiments are useful. This chapter outlines methods to expand, purify, and study the function of mast cells harvested from murine fetal skin. Using these methods, cultured mast cells retain many of the differences in maturity and activation seen during fetal skin development in vivo. Studying the function of mast cells in vitro could help define the mechanisms by which mast cells contribute to wound repair and ultimately lead to better therapies for improving wound repair and reducing scar formation.

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Acknowledgments

The authors are supported in part by NIH grants R01-CA127109 and R21-ES020462.

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Authors and Affiliations

  1. Department of Pathology, The Ohio State University, Columbus, OH, USA

    Brian C. Wulff & Traci A. Wilgus

Authors
  1. Brian C. Wulff
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  2. Traci A. Wilgus
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Editor information

Editors and Affiliations

  1. Virginia Tech Carilion Research Institut, Roanoke, Virginia, USA

    Robert G. Gourdie

  2. School of Pharmacy, MCPHS University, Worcester, Massachusetts, USA

    Tereance A. Myers

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Wulff, B.C., Wilgus, T.A. (2013). Examining the Role of Mast Cells in Fetal Wound Healing Using Cultured Cells In Vitro. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_29

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  • DOI: https://doi.org/10.1007/978-1-62703-505-7_29

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-504-0

  • Online ISBN: 978-1-62703-505-7

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