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Using Drosophila Larvae to Study Epidermal Wound Closure and Inflammation

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Wound Regeneration and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1037))

Abstract

This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, The University of Texas Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Sirisha Burra, Yan Wang, Amanda R. Brock & Michael J. Galko

Authors
  1. Sirisha Burra
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  2. Yan Wang
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  3. Amanda R. Brock
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  4. Michael J. Galko
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Editor information

Editors and Affiliations

  1. Virginia Tech Carilion Research Institut, Roanoke, Virginia, USA

    Robert G. Gourdie

  2. School of Pharmacy, MCPHS University, Worcester, Massachusetts, USA

    Tereance A. Myers

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© 2013 Springer Science+Business Media New York

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Burra, S., Wang, Y., Brock, A.R., Galko, M.J. (2013). Using Drosophila Larvae to Study Epidermal Wound Closure and Inflammation. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_26

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  • DOI: https://doi.org/10.1007/978-1-62703-505-7_26

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-504-0

  • Online ISBN: 978-1-62703-505-7

  • eBook Packages: Springer Protocols

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