Skip to main content
SpringerLink
Log in
Book cover

Mouse Models of Allergic Disease pp 145–158Cite as

Induction of Allergic Rhinitis in Mice

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1032))

Abstract

We describe a method for allergic rhinitis (AR) induction in mice. Methodology involves nasal infusions of small volumes of ovalbumin for both initial sensitization and challenges. The latter are frequent and carried out over several weeks. This methodology more closely resembles natural AR induction than does the common use of systemic sensitization, often with adjuvants, followed by nasal challenges with relatively large allergen volumes. Also described are methodologies for collection of cardiac blood and perfusion for preparation of histological samples, both essential in verifying AR induction in individual animals.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. McCusker C, Chicoine M, Hamid Q, Mazer B (2002) Site-specific sensitization in a murine model of allergic rhinitis: role of the upper airway in lower airways disease. J Allergy Clin Immunol 110:891–898

    Article  PubMed  Google Scholar 

  2. Baroody FM, Naclerio RM (1991) Allergic rhinitis. In: Getchell TV, Doty RL, Bartoshuk LM, Snow JB Jr (eds) Smell and taste in health and disease. Raven, New York, pp 529–552

    Google Scholar 

  3. Apter AJ, Mott AE, Frank ME, Cline JM (1995) Allergic rhinitis and olfactory loss. Ann Allergy Asthma Immunol 75:311–316

    PubMed  CAS  Google Scholar 

  4. Sato J, Asakura K, Murakami M, Uede T, Kaaura A (1999) Topical CTLA4-Ig suppresses ongoing mucosal immune responses in presensitized murine model of allergic rhinitis. Int Arch Allergy Immunol 119:197–204

    Article  PubMed  CAS  Google Scholar 

  5. Saito H, Howie K, Waattie J, Denberg A, Ellis R, Inman MD, Denberg JA (2001) Allergen-induced murine upper airway inflammation: local and systemic changes in murine experimental allergic rhinitis. Immunology 104:226–234

    Article  PubMed  CAS  Google Scholar 

  6. Martin P, Villares R, Rodriguez-Mascarenhas S, Zaballos A, Leitges M, Kovac J, Sizing I, Rennert P, Marquez G, Marinez-A C, Diaz-Meco MT, Moscat J (2005) Control of T helper 2 cell function and allergic airway inflammation by PKCε. Proc Natl Acad Sci USA 102:9866–9871

    Article  PubMed  CAS  Google Scholar 

  7. Rahman A, Yatsuzuka R, Jiang S, Ueda Y, Kamei C (2006) Involvement of cyclooxogenase-2 in allergic inflammation in rats. Int Immunopharmacol 6:1736–1742

    Article  PubMed  CAS  Google Scholar 

  8. Ozaki S, Toida K, Suzuki M, Nakamura Y, Ohno N, Ohasi T, Nakayama M, Hamajima Y, Ingaki A, Kitaoka K, Sei H, Marakami S (2010) Impaired olfactory function in mice with allergic rhinitis. Auris Nasus Larynx 37:575–583

    Article  PubMed  Google Scholar 

  9. van de Rijn M, Mehlhop PD, Judkins A, Rothenberg ME, Luster AD, Oettgen HC (1998) A murine model of allergic rhinitis: studies on the role of IgE in the pathogenesis and analysis of the eosinophil influx elicited by allergen and eotaxin. J Allergy Clin Immunol 74:65–74

    Article  Google Scholar 

  10. Okano M, Nishizaki K, Abe M, Wang M-M, Yoshino T, Satoskar AR, Masuda Y, Harn DA Jr (1999) Strain-dependent induction of allergic rhinitis without adjuvant in mice. Allergy 54:593–601

    Article  PubMed  CAS  Google Scholar 

  11. Epstein VA, Bryce PJ, Conley DB, Robinson AM (2008) Intranasal Aspergillus fumigatus exposure induces eosinophilic inflammation and olfactory sensory neuron cell death in mice. Otolaryngol Head Neck Surg 138:334–339

    Article  PubMed  Google Scholar 

  12. Carr VMcM, Robinson AM, Kern RC (2012) Tissue-specific effects of allergic rhinitis in mouse nasal epithelia. Chem Senses 37:655–668

    Article  PubMed  Google Scholar 

  13. Luna LG (1968) Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York, pp 111–112

    Google Scholar 

Download references

Acknowledgments

The authors express appreciation to Dr. Robert Kern for his advice and support. This research was supported by the Department of Otolaryngology—Head and Neck Surgery, Northwestern University from in-house funds.

Author information

Authors and Affiliations

  1. Department of Otolaryngology, Head & Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA

    Virginia McMillan Carr & Alan M. Robinson

Authors
  1. Virginia McMillan Carr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alan M. Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Dept. of Biomedical Sciences & Pathobiol, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    Irving C. Allen

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Carr, V.M., Robinson, A.M. (2013). Induction of Allergic Rhinitis in Mice. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-496-8_12

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-495-1

  • Online ISBN: 978-1-62703-496-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation