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Transplantation Immunology pp 385–403Cite as

RNA Purification and Expression Analysis Using Microarrays and RNA Deep Sequencing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

Abstract

Transcriptome analysis or global gene expression profiling is a powerful tool for discovery as well as ­understanding biological mechanisms in health and disease. We present in this chapter a description of methods used to isolate mRNA from cells and tissues that has been optimized for preservation of RNA quality using clinical materials and implemented successfully in several large, multicenter studies by the authors. In addition, two methods, gene expression microarrays and RNAseq, are described for mRNA profiling of cells and tissues from clinical or laboratory sources.

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References

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Author information

Authors and Affiliations

  1. Microarray and Next Generation Sequencing Core Facility, The Scripps Research Institute, La Jolla, CA, USA

    Steven R. Head, Tony Mondala, Phillip Ordoukhanian, Rebecca Chappel & Gilberto Hernandez

  2. Laboratory for Functional Genomics, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA

    Terri Gelbart & Daniel R. Salomon

Authors
  1. Steven R. Head
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  2. Tony Mondala
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  3. Terri Gelbart
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  4. Phillip Ordoukhanian
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  5. Rebecca Chappel
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  6. Gilberto Hernandez
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  7. Daniel R. Salomon
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Editor information

Editors and Affiliations

  1. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Andrea A. Zachary

  2. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Mary S. Leffell

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© 2013 Springer Science+Business Media, LLC

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Cite this protocol

Head, S.R. et al. (2013). RNA Purification and Expression Analysis Using Microarrays and RNA Deep Sequencing. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_25

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

  • eBook Packages: Springer Protocols

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